Method validation for the analysis of estrogens ( including conjugated compounds ) in various aqueous matrices
نویسندگان
چکیده
Estrogens act as endocrine disruptors and are known to be discharged in the aquatic environment via effluents from wastewater treatment plants. Efficient analytical protocols are needed to identify and quantify estrogens in wastewaters and aquatic environments in order to study their sources, levels of exposure and modes of transfer. We developed and validated a new analytical method for estrogens in water. It allows to quantify five estrogenic hormones and their conjugated forms at the ng/L level in rivers, wastewater treatment plant influents and effluents, with a satisfying precision (relative standard deviation of within-day analytical recoveries usually lower than 22%). The method was statistically proven to be specific, thanks to the use of perdeuterated hormones as internal surrogates, which proved to be an efficient means to correct for matrix effects.
منابع مشابه
Method validation for the analysis of estrogens (including conjugated compounds) in aqueous matrices
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